Automatic alignment based on gray values
• Selected regions
• Resampled version
• Within restricted limits
• With selectable accuracy
• One one selected channel (in case of images with different independent channels)
• RGB-option for color images
• Quality calculation
Manual alignment
• Real-time interaction: shifting and rotating layers
• Fine-tuning via arrow-keys
• Different overlay modes (gray, colored)
• Quality calculation
Pre-processing steps
• Normalization
• Inverting gray values
• Layer manager (IRIX)
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Post-processing steps
• Interpolation of bad layer
• Cropping common region
• Saving alignment information
File formats
• IMARIS format and TIFF series
• Virtually all formats used in confocal micrcoscopy, optical microscopy and electron micrsocopy (IRIX)
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