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Opening The Door To 3D Electron Microscopy

Microtomy is a standard technique used for 2D microscopy, but it can also provide the foundation for 3D analysis using AutoAligner. AutoAligner reads a stack of serial sections and automatically aligns the single images, building up a 3D space that can be explored using Imaris. Each image is aligned in translation and rotation relative to its precursor image in the sequence. In addition to a fully automated mode, AutoAligner also provides manual control for neighboring images that are too different from each other for automatic alignment. The visual alignment tool uses fast display techniques for aligning images in real time.

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